The influence of calyculin A on lymphocytes in vitro.
J Appl Genet
; 44(3): 413-8, 2003.
Article
in En
| MEDLINE
| ID: mdl-12923316
ABSTRACT
Human lymphocytes were cultured in vitro and treated with calyculin A. The aim of this work was to estimate the influence of calyculin A on chromosome morphology and banding patterns. It was also interesting whether calyculin A treatment is useful in cytogenetic analysis of human karyotype. We proved that calyculin A induces chromosome condensation in lymphocytes and raises the mitotic index significantly. Moreover, calyculin A does not influence the banding patterns. Therefore it is concluded that calyculin A can be clinically useful for human karyotyping.
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Database:
MEDLINE
Main subject:
Oxazoles
/
Lymphocytes
/
Chromosomes
/
Phosphoprotein Phosphatases
Limits:
Humans
Language:
En
Year:
2003
Type:
Article