High-level expression in Escherichia coli and rapid purification of enzymatically active honey bee venom phospholipase A2.

Dudler, T; Chen, W Q; Wang, S; Schneider, T; Annand, R R; Dempcy, R O; Crameri, R; Gmachl, M; Suter, M; Gelb, M H.

Affiliation

Biochim Biophys Acta ; 1165(2): 201-10, 1992 Dec 02.

Article in En | MEDLINE | ID: mdl-1450215

Subject(s)

Bee Venoms/genetics; Phospholipases A/genetics; Amino Acid Sequence; Base Sequence; Bee Venoms/enzymology; Chromatography, Ion Exchange; Cloning, Molecular; DNA; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Genes, Synthetic; Kallikreins/metabolism; Kinetics; Molecular Sequence Data; Phospholipases A/chemistry; Phospholipases A/isolation & purification; Phospholipases A/metabolism; Phospholipases A2; Plasmids; Protein Folding; Recombinant Fusion Proteins/genetics; Recombinant Fusion Proteins/isolation & purification; Recombinant Fusion Proteins/metabolism; Structure-Activity Relationship

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Database: MEDLINE Main subject: Phospholipases A / Bee Venoms Language: En Year: 1992 Type: Article

Database: MEDLINE Main subject: Phospholipases A / Bee Venoms Language: En Year: 1992 Type: Article