Efficient treatment of paraffin-embedded cervical tissue for HPV DNA testing by HC-II and PCR assays.
J Clin Virol
; 29(2): 137-40, 2004 Feb.
Article
in En
| MEDLINE
| ID: mdl-14747034
ABSTRACT
BACKGROUND AND OBJECTIVES:
High-risk human papillomaviruses (HR-HPVs) are the primary cause of cervical cancer. In order to meet with clinical requirements, a direct capture test with signal amplification (HC-II), able to detect the 13 prevalent HR-HPVs, has been developed and validated for cytological specimens. STUDYDESIGN:
the use of HC-II assay with formaldehyde-fixed paraffin-embedded cervical biopsies, for retrospective studies or to support histological findings, was investigated by analysing three different sample treatments. The efficacy of this test was compared with a reference PCR-ELISA, using MY09/11 and GP5+/6+ consensus primers and the use of a single extraction method for both HC-II and PCR-ELISA assays was validated.RESULTS:
protease treatment of dewaxed biopsy sections allowed an optimal performance of HC-II and has also been validated for PCR-ELISA. Overall, on the analysis of 50 cervical samples HC-II and PCR-ELISA assays showed a high concordance (K=0.80). Compared with PCR-ELISA, the HC-II had a sensitivity of 86.4% and a specificity of 92.9%. The results showed that short amplimers are necessary for a sensitive PCR-ELISA from formaldehyde-fixed paraffin-embedded biopsies, while HC-II showed a relatively low sensitivity for HPV18 within the HR probe pool.CONCLUSIONS:
HC-II can be a valid tool for the diagnosis of HPV infection in biopsy material. The possibility to use the same specimen preparation material for both HC-II and PCR-ELISA allows HC-II positive specimens to be further processed by PCR-ELISA if specific genotyping is needed.
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Database:
MEDLINE
Main subject:
Papillomaviridae
/
Tissue Preservation
/
DNA, Viral
/
Cervix Uteri
/
Paraffin Embedding
/
Nucleic Acid Hybridization
Type of study:
Diagnostic_studies
/
Evaluation_studies
/
Observational_studies
/
Risk_factors_studies
Limits:
Female
/
Humans
Language:
En
Year:
2004
Type:
Article