Carboxyfluorescein diacetate succinimidyl ester fluorescent dye for cell labeling.
Acta Biochim Biophys Sin (Shanghai)
; 37(6): 379-85, 2005 Jun.
Article
in En
| MEDLINE
| ID: mdl-15944752
ABSTRACT
Our objective was to study the properties of the carboxyfluorescein diacetate succinimidyl ester (CFDA-SE) and the methodology of cell labeling using CFDA-SE fluorescent dye. First, we analyzed the kinetics of CFDA-SE fluorescent dye intensity over time. Second, we determined the optimal concentration of CFDA-SE fluorescent dye for cell labeling. Third, we tested the toxicity of CFDA-SE fluorescent dye on labeled cells. Finally, we determined the optimal staining time of CFDA-SE fluorescent dye for cell labeling. The results show that the optimal concentration of CFDA-SE fluorescent dye for cell labeling varies according to different cell types. CFDA-SE fluorescent dye is non-toxic to cells as the cell death rate caused by CFDA-SE labeling is below 5%. The optimal cell labeling time was determined to be 8 min of incubation with CFDA-SE fluorescent dye. We concluded that the advantages of using CFDA-SE fluorescent dye for cell labeling are as follows (1) the binding of CFDA-SE fluorescent dye to cells is stable; (2) CFDA-SE fluorescent dye is not toxic and does not modify the viability of labeled cells; and (3) CFDA-SE fluorescent dye is a suitable fluorochrome for cell labeling.
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Database:
MEDLINE
Main subject:
Staining and Labeling
/
Succinimides
/
Cytological Techniques
/
Fluoresceins
/
Fluorescent Dyes
Limits:
Animals
/
Humans
Language:
En
Year:
2005
Type:
Article