Metagenomics to paleogenomics: large-scale sequencing of mammoth DNA.
Science
; 311(5759): 392-4, 2006 Jan 20.
Article
in En
| MEDLINE
| ID: mdl-16368896
ABSTRACT
We sequenced 28 million base pairs of DNA in a metagenomics approach, using a woolly mammoth (Mammuthus primigenius) sample from Siberia. As a result of exceptional sample preservation and the use of a recently developed emulsion polymerase chain reaction and pyrosequencing technique, 13 million base pairs (45.4%) of the sequencing reads were identified as mammoth DNA. Sequence identity between our data and African elephant (Loxodonta africana) was 98.55%, consistent with a paleontologically based divergence date of 5 to 6 million years. The sample includes a surprisingly small diversity of environmental DNAs. The high percentage of endogenous DNA recoverable from this single mammoth would allow for completion of its genome, unleashing the field of paleogenomics.
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Database:
MEDLINE
Main subject:
Paleontology
/
Sequence Analysis, DNA
/
Genomics
/
Elephants
/
Fossils
/
Mandible
Limits:
Animals
/
Humans
Country/Region as subject:
Asia
Language:
En
Year:
2006
Type:
Article