Using PCR to extend the limit of oligonucleotide synthesis.
Biotechniques
; 12(1): 44, 46, 48, 1992 Jan.
Article
in En
| MEDLINE
| ID: mdl-1734921
ABSTRACT
A method has been developed to allow one to extend the practical limit of oligonucleotide synthesis by coupling the synthesis reaction to a subsequent PCR. Given that DNA synthesizers are capable of producing reasonable yields of oligonucleotides that are 125-150 bases in length, this method could be used to recover the minute amount of full-length product present in mixtures extended well beyond the established limits. This technology could be applied to gene synthesis and mutagenesis.
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Database:
MEDLINE
Main subject:
Oligonucleotides
/
Polymerase Chain Reaction
Language:
En
Year:
1992
Type:
Article