Development of recombinant nucleocapsid protein based IgM-ELISA for the early detection of distemper infection in dogs.
Vet Immunol Immunopathol
; 119(3-4): 278-86, 2007 Oct 15.
Article
in En
| MEDLINE
| ID: mdl-17659785
ABSTRACT
An IgM-ELISA based on a 16-kDa recombinant protein produced for the conserved and functional middle region of nucleocapsid protein of Canine distemper virus was developed. Out of 70 serum samples from distemper-suspected and vaccinated dogs analyzed, 34 serum samples (49%) were positive. The specificity of this ELISA was confirmed by blocking and adsorption experiments. The IgM-ELISA based on the recombinant nucleocapsid protein showed a strong correlation (r=0.857, p<0.0001 at 95% CI) and good agreement (kappa=0.714) with the conventional Vero cell culture distemper antigen based IgM-ELISA. The percent positivity was more in dogs with systemic signs (62%) by recombinant nucleocapsid protein IgM-ELISA. Out of 70 clinical serum samples, 69 samples were used along with 4 control sera used in the IgM-ELISA for the detection of viral RNA by Slot blot hybridization and 26 of them (36%) were positive. Fifty-one percent agreement was observed between the recombinant nucleocapsid protein IgM-ELISA and Slot blot hybridization. The analysis of clinical history of the dogs with systemic signs supported the application of IgM-ELISA over Slot blot hybridization in the early detection of distemper infection.
Search on Google
Database:
MEDLINE
Main subject:
Recombinant Proteins
/
Immunoglobulin M
/
Enzyme-Linked Immunosorbent Assay
/
Nucleocapsid Proteins
/
Distemper
/
Distemper Virus, Canine
Type of study:
Diagnostic_studies
/
Screening_studies
Limits:
Animals
Language:
En
Year:
2007
Type:
Article