A soluble and active form of Wnt-3a protein is involved in myogenic differentiation after cholesterol depletion.
FEBS Lett
; 581(30): 5787-95, 2007 Dec 22.
Article
in En
| MEDLINE
| ID: mdl-18037380
ABSTRACT
Cholesterol is one of the major lipids of plasma membranes. Recently, we have shown that cholesterol depletion by methyl-beta-cyclodextrin (M beta CD) induces the activation of the Wnt/beta-catenin pathway and enhances myogenic differentiation. Here, we show that M beta CD-conditioned media accelerates myogenesis in a similar way as M beta CD does, suggesting that the effects induced by M beta CD could be caused by soluble factors present in the culture medium. Soluble Wnt-3 protein is significantly enhanced in M beta CD-conditioned medium. Wnt-3a-enriched media induces myogenesis as much as M beta CD does, whereas Wnt-5a-enriched media inhibits. We suggest that Wnt-3a is involved in the myogenic induction observed after cholesterol depletion.
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Database:
MEDLINE
Main subject:
Cell Differentiation
/
Cholesterol
/
Muscle Development
/
Muscle Cells
/
Wnt Proteins
Limits:
Animals
/
Humans
Language:
En
Year:
2007
Type:
Article