Validation of a Tn5 transposon mutagenesis system for Gluconacetobacter diazotrophicus through characterization of a flagellar mutant.
Arch Microbiol
; 189(4): 397-405, 2008 Apr.
Article
in En
| MEDLINE
| ID: mdl-18060666
ABSTRACT
Gluconacetobacter diazotrophicus is a nitrogen-fixing bacterium, which was originally isolated from the interior of sugarcane plants. The genome of strain PAL5 of G. diazotrophicus has been completely sequenced and a next step is the functional characterization of its genes. The aim of this study was to establish an efficient mutagenesis method, using the commercial Tn5 transposon EZTn5
Search on Google
Database:
MEDLINE
Main subject:
DNA Transposable Elements
/
Mutagenesis, Insertional
/
Acetobacteraceae
/
Flagella
Language:
En
Year:
2008
Type:
Article