Gingival fibroblasts inhibit MMP-1 and MMP-3 activities in an ex-vivo artery model.
Connect Tissue Res
; 48(6): 300-8, 2007.
Article
in En
| MEDLINE
| ID: mdl-18075816
ABSTRACT
The main arterial pathologies can be associated with a deregulation of remodeling involving matrix metalloproteinases (MMPs), whereas gingival healing is characterized by an absence of fibrosis or irreversible elastin/collagen degradation. The aim of our study was to evaluate the effect of gingival fibroblasts on MMP-1 and MMP-3 secretion in an organotypic artery culture. MMP-1 and MMP-3 secretions and activities (dot blots, zymography, ELISA) were evaluated in coculture of rabbit artery in the presence or not of gingival fibroblasts. MMP-1/TIMP-1 and MMP-3/TIMP-1 complexes forms were measured by ELISA. Complementary studies were performed using human aortic smooth muscle cells cocultured with adventitial, dermal, or gingival fibroblasts. Our results indicated that MMP-1 and MMP-3 free-forms activities were significantly reduced in coculture. This inhibition was linked to a significant increase of TIMP-1 leading to formation of TIMP-1/MMPs complexes. Due to the presence of gingival fibroblasts, the decrease in MMP-1 and MMP-3 efficiency thus contributes to diminish the degradation of artery. This cellular therapy strategy could be promising in artery pathologies treatment.
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Database:
MEDLINE
Main subject:
Tissue Inhibitor of Metalloproteinase-1
/
Fibroblasts
/
Matrix Metalloproteinase Inhibitors
/
Gingiva
Limits:
Adult
/
Animals
/
Humans
Language:
En
Year:
2007
Type:
Article