Proteomic analysis of RNA-binding proteins in dry seeds of rice after fractionation by ssDNA affinity column chromatography.
Biotechnol Lett
; 30(5): 955-60, 2008 May.
Article
in En
| MEDLINE
| ID: mdl-18085409
ABSTRACT
In proteomic analysis, one of the major limitations is the detection of low-abundance proteins. To detect low-abundance RNA-binding proteins in mature dry seeds of rice, fractionation by single stranded DNA (ssDNA) affinity column chromatography was carried out before analysis by two-dimensional gel electrophoresis (2-DE). Proteomic analysis of the ssDNA-binding fraction revealed the existence of three types of RNA-binding proteins, including a K homology (KH) domain containing protein, a putative RNA-binding protein and a glycine-rich RNA-binding protein, in mature seeds. In addition, decreases in the putative RNA-binding protein and glycine-rich RNA-binding protein after absorbing water in seeds appear to be associated with seed germination.
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Database:
MEDLINE
Main subject:
Oryza
/
Seeds
/
RNA-Binding Proteins
/
Proteomics
Language:
En
Year:
2008
Type:
Article