Semi-automated microseeding of nanolitre crystallization experiments.
Acta Crystallogr Sect F Struct Biol Cryst Commun
; 64(Pt 1): 14-8, 2008 Jan 01.
Article
in En
| MEDLINE
| ID: mdl-18097093
ABSTRACT
A simple semi-automated microseeding procedure for nanolitre crystallization experiments is described. Firstly, a microseed stock solution is made from microcrystals using a Teflon bead. A dilution series of this microseed stock is then prepared and dispensed as 100 nl droplets into 96-well crystallization plates, facilitating the incorporation of seeding into high-throughput crystallization pipelines. This basic microseeding procedure has been modified to include additive-screening and cross-seeding methods. Five examples in which these techniques have been used successfully are described.
Full text:
1
Database:
MEDLINE
Main subject:
Bacterial Proteins
/
Viral Proteins
/
Nanotechnology
Language:
En
Year:
2008
Type:
Article