Pathways change in expression during replicative aging in Saccharomyces cerevisiae.
J Gerontol A Biol Sci Med Sci
; 63(1): 21-34, 2008 Jan.
Article
in En
| MEDLINE
| ID: mdl-18245757
ABSTRACT
Yeast replicative aging is a process resembling replicative aging in mammalian cells. During aging, wild-type haploid yeast cells enlarge, become sterile, and undergo nucleolar enlargement and fragmentation; we sought gene expression changes during the time of these phenotypic changes. Gene expression studied via microarrays and quantitative real-time reverse-transcription polymerase chain reaction (qPCR) has shown reproducible, statistically significant changes in messenger RNA (mRNA) of genes at 12 and 18-20 generations. Our findings support previously described changes towards aerobic metabolism, decreased ribosome gene expression, and a partial environmental stress response. Our findings include a pseudostationary phase, downregulation of methylation-related metabolism, increased nucleotide excision repair-related mRNA, and a strong upregulation of many of the regulatory subunits of protein phosphatase I (Glc7). These findings are correlated with aging changes in higher organisms as well as with the known involvement of protein phosphorylation states during yeast aging.
Full text:
1
Database:
MEDLINE
Main subject:
Saccharomyces cerevisiae
/
Aging
/
RNA, Messenger
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Gene Expression Regulation, Fungal
Language:
En
Year:
2008
Type:
Article