Molecular characterization of inflammation and Staphylococcus aureus colonization of involved skin of atopic dermatitis patients. A non-invasive approach.
Skin Pharmacol Physiol
; 21(5): 260-8, 2008.
Article
in En
| MEDLINE
| ID: mdl-18612217
ABSTRACT
Atopic dermatitis (AD) is a multifactorial chronic inflammatory disease mainly stemming from a genetic predisposition that leads to hypersensitivity to environmental factors and a common involvement of Staphylococcus aureus (SA) colonization. The aim of this work was to propose a new non-invasive approach to enumerate the genes coding for the toxins of SA in atopic skin samples. In parallel, the study aimed to evaluate the change in AD through 3 markers of the inflammatory response IL-8, IL-1RA/IL-1alpha and IL-18. These methods were tested on 31 patients with AD, and finally on a group of 19 subjects for whom clinical improvement had been reported after various treatments. The study revealed the presence of a large number of genes encoding toxins in atopic samples, indicating a high rate of SA colonization, and also an increase in the level of all cytokine markers in atopic skin compared to the skin of healthy subjects. Finally, we found a positive correlation between increases in the SCORAD (Scoring Atopic Dermatitis Index) value after treatment and the corresponding evolution of the SA density. These methods provide a means to clinically evaluate the course of AD, and may help in the development of potential treatments.
Full text:
1
Database:
MEDLINE
Main subject:
Staphylococcus aureus
/
Bacterial Toxins
/
Polymerase Chain Reaction
/
Dermatitis, Atopic
Type of study:
Observational_studies
Limits:
Child
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Child, preschool
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Humans
/
Infant
Language:
En
Year:
2008
Type:
Article