mda-7/IL-24 induces apoptosis in human HepG2 hepatoma cells by endoplasmic reticulum stress.
Oncol Rep
; 20(2): 437-42, 2008 Aug.
Article
in En
| MEDLINE
| ID: mdl-18636209
ABSTRACT
mda-7/IL-24 shows tumor-suppressor activity in a broad spectrum of human cancer cells. However, the molecular mechanism by which mda-7/IL-24 induces apoptosis is not well understood and most likely involves different pathways depending on the tumor. We examined the apoptotic effect of the adenovirus-mediated mda-7/IL-24 (Ad.mda-7) on human HepG2 hepatoma cells. We found that blocking the endoplasmic reticulum (ER) stress inhibited apoptosis induced by Ad.mda-7 and down-regulated the expression of caspase-12, Bax and caspase-3. The treatment of subcutaneous tumor xenografts of HepG2 cells with Ad.mda-7 inhibited tumor growth and angiogenesis. As in the in vitro studies, we found that blocking ER stress prevented Ad.mda-7 from inducing apoptosis in liver cancer cells in vivo. Our studies suggest that Ad.mda-7 induces apoptosis of HepG2 cells mainly through activation of the ER stress pathway.
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Database:
MEDLINE
Main subject:
Genetic Therapy
/
Interleukins
/
Apoptosis
/
Carcinoma, Hepatocellular
/
Endoplasmic Reticulum
/
Liver Neoplasms, Experimental
Limits:
Animals
/
Humans
Language:
En
Year:
2008
Type:
Article