Antibody like activities against cholesterol ester in anti-LDL antiserum. An observation on the surface of LDL.

ABSTRACT

The antibody like activities against cholesterol and cholesterol ester were examined by means of radioimmunoassay system, immunolysis of liposomes containing cholesterol and other steroids. In comparison with anti-etiocholenic acid antiserum and anti-cholesterol succinate antiserum, it was proved that anti-LDL antiserum contained 2 kinds of antibodies, one was specific to the structure of cholesterol skeleton and other was specific to the whole structure of cholesterol ester. LDL only combined with anti-cholesterol succinate antibody resulting with the inhibition of the interaction between sheep red cell sensitized by cholesterol succinate and anti-cholesterol succinate antiserum by means of passive hemagglutionation reaction.