In vitro reconstitution of activation of PLCepsilon by Ras and Rho GTPases.

Gandarillas, Natalia Lamuño; Bunney, Tom D; Josephs, Michelle B; Gierschik, Peter; Katan, Matilda

Gandarillas, Natalia Lamuño; Bunney, Tom D; Josephs, Michelle B; Gierschik, Peter; Katan, Matilda.

Affiliation

Gandarillas NL; Cancer Research UK Centre for Cell and Molecular Biology, Chester Beatty Laboratories, The Institute of Cancer Research, London, UK.

Methods Mol Biol ; 462: 379-89, 2009.

Article in En | MEDLINE | ID: mdl-19160682

Subject(s)

Baculoviridae/enzymology; Phosphoinositide Phospholipase C/isolation & purification; Phosphoinositide Phospholipase C/metabolism; ras Proteins/isolation & purification; ras Proteins/pharmacology; rho GTP-Binding Proteins/isolation & purification; rho GTP-Binding Proteins/pharmacology; Animals; Cell Line; Cell Membrane/chemistry; Cell-Free System; Chromatography, Affinity; Chromatography, Gel; Enzyme Activation/drug effects; Escherichia coli/cytology; Isoenzymes/genetics; Isoenzymes/isolation & purification; Isoenzymes/metabolism; Phosphoinositide Phospholipase C/genetics; Rats; Sequence Deletion; Solubility

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Database: MEDLINE Main subject: Baculoviridae / Ras Proteins / Rho GTP-Binding Proteins / Phosphoinositide Phospholipase C Limits: Animals Language: En Year: 2009 Type: Article

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