Differential DNA binding by monomeric, homodimeric, and potentially heteromeric forms of the thyroid hormone receptor.
Mol Cell Biol
; 11(10): 5005-15, 1991 Oct.
Article
in En
| MEDLINE
| ID: mdl-1922030
ABSTRACT
Binding of the thyroid hormone receptor (TR) to thyroid hormone-responsive elements (TREs) is crucial for regulation of gene expression by thyroid hormone. The TR binds to each half-site of a palindromic TRE separately, as a monomer, or simultaneously, as a homodimer. In addition, the TR monomer interacts with a 42-kDa protein that may be responsible for an increase in the apparent size and stability of the TR-TRE complex after incubation with liver nuclear extract. The multiple DNA-binding forms of the TR contact the TRE differently but compete for binding in a dynamic equilibrium which is highly dependent on the relative concentrations of TR and nuclear protein. Thus, protein-protein interactions are likely to determine the context in which the TR binds to target genes and regulates the transcriptional response to thyroid hormone.
Full text:
1
Database:
MEDLINE
Main subject:
Receptors, Thyroid Hormone
/
DNA
/
Regulatory Sequences, Nucleic Acid
Limits:
Animals
Language:
En
Year:
1991
Type:
Article