Purification, characterization, molecular cloning, and expression of a new aminoacylase from Streptomyces mobaraensis that can hydrolyze N-(middle/long)-chain-fatty-acyl-L-amino acids as well as N-short-chain-acyl-L-amino acids.

Koreishi, Mayuko; Nakatani, Yasuyuki; Ooi, Manami; Imanaka, Hiroyuki; Imamura, Koreyoshi; Nakanishi, Kazuhiro

Koreishi, Mayuko; Nakatani, Yasuyuki; Ooi, Manami; Imanaka, Hiroyuki; Imamura, Koreyoshi; Nakanishi, Kazuhiro.

Affiliation

Koreishi M; Division of Chemistry and Biochemistry, Graduate School of Natural Science and Technology, Okayama University, 3-1-1 Tsushima-naka, Okayama 700-8530, Japan.

Biosci Biotechnol Biochem ; 73(9): 1940-7, 2009 Sep.

Article in En | MEDLINE | ID: mdl-19734688

Subject(s)

Amidohydrolases/isolation & purification; Amino Acids/metabolism; Fatty Acids/metabolism; Streptomyces/enzymology; Amidohydrolases/chemistry; Amidohydrolases/genetics; Amino Acid Sequence; Base Sequence; Chromatography, Ion Exchange; Cloning, Molecular; Culture Media; DNA Primers; Electrophoresis, Polyacrylamide Gel; Hydrolysis; Molecular Sequence Data; Polymerase Chain Reaction; Sequence Homology, Amino Acid

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