Localization and polymorphism of a chromosome 12-specific alpha satellite DNA sequence.
Cytogenet Cell Genet
; 53(4): 216-8, 1990.
Article
in En
| MEDLINE
| ID: mdl-1976486
ABSTRACT
The isolation and localization of a chromosome 12-specific alpha satellite DNA sequence, p alpha 12H8, is described. This clone contains a complete copy of the 1.4-kb HindIII higher-order repeat present within the alpha satellite array on chromosome 12. The specificity of p alpha 12H8 was demonstrated by in situ hybridization and Southern blot analysis of a somatic cell hybrid mapping panel, both performed under high-stringency conditions. Polymorphic restriction patterns within the alpha satellite array, revealed by the use of the restriction enzymes BglII and EcoRV, were demonstrated to display Mendelian inheritance. These properties make p alpha 12H8 a valuable genetic marker for the centromeric region of chromosome 12.
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Database:
MEDLINE
Main subject:
Chromosomes, Human, Pair 12
/
Polymorphism, Restriction Fragment Length
/
DNA, Satellite
/
Centromere
Limits:
Humans
Language:
En
Year:
1990
Type:
Article