Expression, purification, characterization and crystallization of non- and phosphorylated states of JAK2 and JAK3 kinase domain.

Hall, Troii; Emmons, Thomas L; Chrencik, Jill E; Gormley, Jennifer A; Weinberg, Robin A; Leone, Joseph W; Hirsch, Jeffrey L; Saabye, Matthew J; Schindler, John F; Day, Jacqueline E; Williams, Jennifer M; Kiefer, James R; Lightle, Sandra A; Harris, Melissa S; Guru, Siradanahalli; Fischer, H David; Tomasselli, Alfredo G

Hall, Troii; Emmons, Thomas L; Chrencik, Jill E; Gormley, Jennifer A; Weinberg, Robin A; Leone, Joseph W; Hirsch, Jeffrey L; Saabye, Matthew J; Schindler, John F; Day, Jacqueline E; Williams, Jennifer M; Kiefer, James R; Lightle, Sandra A; Harris, Melissa S; Guru, Siradanahalli; Fischer, H David; Tomasselli, Alfredo G.

Affiliation

Hall T; Pfizer Inc., Global Research and Development, St. Louis Laboratories, St. Louis, MO 63017-1732, USA.

Protein Expr Purif ; 69(1): 54-63, 2010 Jan.

Article in En | MEDLINE | ID: mdl-19781647

Subject(s)

Janus Kinase 2/chemistry; Janus Kinase 2/isolation & purification; Janus Kinase 3/chemistry; Janus Kinase 3/isolation & purification; Amino Acid Sequence; Biocatalysis; Chromatography, High Pressure Liquid; Crystallization; Electrophoresis, Polyacrylamide Gel; Fermentation; Humans; Kinetics; Molecular Sequence Data; Phosphorylation; Protein Structure, Tertiary

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Full text: 1 Database: MEDLINE Main subject: Janus Kinase 2 / Janus Kinase 3 Type of study: Guideline Limits: Humans Language: En Year: 2010 Type: Article

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Full text: 1 Database: MEDLINE Main subject: Janus Kinase 2 / Janus Kinase 3 Type of study: Guideline Limits: Humans Language: En Year: 2010 Type: Article