Use of thermal melt curves to assess the quality of enzyme preparations.

Crowther, Gregory J; He, Panqing; Rodenbough, Philip P; Thomas, Andrew P; Kovzun, Kuzma V; Leibly, David J; Bhandari, Janhavi; Castaneda, Lisa J; Hol, Wim G J; Gelb, Michael H; Napuli, Alberto J; Van Voorhis, Wesley C

Crowther, Gregory J; He, Panqing; Rodenbough, Philip P; Thomas, Andrew P; Kovzun, Kuzma V; Leibly, David J; Bhandari, Janhavi; Castaneda, Lisa J; Hol, Wim G J; Gelb, Michael H; Napuli, Alberto J; Van Voorhis, Wesley C.

Affiliation

Crowther GJ; Department of Medicine, University of Washington, Seattle, WA 98195, USA. crowther@uw.edu

Anal Biochem ; 399(2): 268-75, 2010 Apr 15.

Article in En | MEDLINE | ID: mdl-20018159

Subject(s)

Enzymes/chemistry; Reverse Transcriptase Polymerase Chain Reaction/methods; Animals; Cattle; Cysteine Proteases/chemistry; Entamoeba histolytica/enzymology; Enzyme Assays; Fluorescent Dyes/chemistry; Hot Temperature; Phase Transition; Plasmodium/enzymology; Protein Denaturation; Sodium Dodecyl Sulfate/chemistry; Xanthine Oxidase/chemistry

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Full text: 1 Database: MEDLINE Main subject: Reverse Transcriptase Polymerase Chain Reaction / Enzymes Limits: Animals Language: En Year: 2010 Type: Article

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Full text: 1 Database: MEDLINE Main subject: Reverse Transcriptase Polymerase Chain Reaction / Enzymes Limits: Animals Language: En Year: 2010 Type: Article