A novel (18)O inverse labeling-based workflow for accurate bottom-up mass spectrometry quantification of proteins separated by gel electrophoresis.

Santos, Hugo M; Glez-Peña, Daniel; Reboiro-Jato, Miguel; Fdez-Riverola, Florentino; Diniz, Mário S; Lodeiro, Carlos; Capelo-Martínez, José-Luis

Santos, Hugo M; Glez-Peña, Daniel; Reboiro-Jato, Miguel; Fdez-Riverola, Florentino; Diniz, Mário S; Lodeiro, Carlos; Capelo-Martínez, José-Luis.

Affiliation

Santos HM; REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, FCT, Universidade Nova de Lisboa, Caparica, Portugal.

Electrophoresis ; 31(20): 3407-19, 2010 Oct.

Article in En | MEDLINE | ID: mdl-20882554

Subject(s)

Electrophoresis, Polyacrylamide Gel/methods; Isotope Labeling/methods; Oxygen Isotopes/analysis; Proteins/analysis; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods; Animals; Cattle; Enzyme-Linked Immunosorbent Assay; Fishes; Models, Chemical; Oxygen Isotopes/metabolism; Peptide Fragments/analysis; Peptide Fragments/metabolism; Proteins/metabolism; Reproducibility of Results; Statistics, Nonparametric; Trypsin/metabolism

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Full text: 1 Database: MEDLINE Main subject: Oxygen Isotopes / Proteins / Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Electrophoresis, Polyacrylamide Gel / Isotope Labeling Type of study: Prognostic_studies Limits: Animals Language: En Year: 2010 Type: Article

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