Screening of genetic parameters for soluble protein expression in Escherichia coli.

Vernet, Erik; Kotzsch, Alexander; Voldborg, Bjørn; Sundström, Michael

Vernet, Erik; Kotzsch, Alexander; Voldborg, Bjørn; Sundström, Michael.

Affiliation

Vernet E; The Novo Nordisk Foundation Center for Protein Research, Faculty of Health Sciences, University of Copenhagen, 2200 Copenhagen N, Denmark. erik.vernet@cpr.ku.dk

Protein Expr Purif ; 77(1): 104-11, 2011 May.

Article in En | MEDLINE | ID: mdl-21130169

Subject(s)

Escherichia coli/genetics; Escherichia coli/metabolism; Protein Engineering/methods; Recombinant Fusion Proteins/biosynthesis; Recombinant Fusion Proteins/genetics; Cloning, Molecular; Codon; Disulfides; Electrophoresis, Polyacrylamide Gel; Escherichia coli Proteins/biosynthesis; Escherichia coli Proteins/chemistry; Escherichia coli Proteins/genetics; Humans; Intercellular Signaling Peptides and Proteins/biosynthesis; Intercellular Signaling Peptides and Proteins/chemistry; Intercellular Signaling Peptides and Proteins/genetics; Interleukins/biosynthesis; Interleukins/chemistry; Interleukins/genetics; Plasmids; Recombinant Fusion Proteins/chemistry; Reproducibility of Results; Solubility; Surface Plasmon Resonance

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Full text: 1 Database: MEDLINE Main subject: Recombinant Fusion Proteins / Protein Engineering / Escherichia coli Type of study: Diagnostic_studies / Screening_studies Limits: Humans Language: En Year: 2011 Type: Article

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