DNA-templated protein arrays for single-molecule imaging.
Nano Lett
; 11(2): 657-60, 2011 Feb 09.
Article
in En
| MEDLINE
| ID: mdl-21218848
ABSTRACT
Single-particle electron cryomicroscopy permits structural characterization of noncrystalline protein samples, but throughput is limited by problems associated with sample preparation and image processing. Three-dimensional density maps are reconstructed from high resolution but noisy images of individual molecules. We show that self-assembled DNA nanoaffinity templates can create dense, nonoverlapping arrays of protein molecules, greatly facilitating data collection. We demonstrate this technique using a G-protein-coupled membrane receptor, a soluble G-protein, and a signaling complex of both molecules.
Full text:
1
Database:
MEDLINE
Main subject:
DNA
/
Molecular Probe Techniques
/
Nanotechnology
/
Protein Array Analysis
Language:
En
Year:
2011
Type:
Article