Modular construction of multifunctional bioresponsive cell-targeted nanoparticles for gene delivery.
Bioconjug Chem
; 22(2): 156-68, 2011 Feb 16.
Article
in En
| MEDLINE
| ID: mdl-21222431
ABSTRACT
Multifunctional and modular block copolymers prepared from biocompatible monomers and linked by a bioreducible disulfide linkage have been prepared using a combination of ring-opening and atom-transfer radical polymerizations (ATRP). The presence of terminal functionality via ATRP allowed cell-targeting folic acid groups to be attached in a controllable manner, while the block copolymer architecture enabled well-defined nanoparticles to be prepared by a water-oil-water double emulsion procedure to encapsulate DNA with high efficiency. Gene delivery assays in a Calu-3 cell line indicated specific folate-receptor-mediated uptake of the nanoparticles, and triggered release of the DNA payload via cleavage of the disulfide link resulted in enhanced transgene expression compared to nonbioreducible analogues. These materials offer a promising and generic means to deliver a wide variety of therapeutic payloads to cells in a selective and tunable way.
Full text:
1
Database:
MEDLINE
Main subject:
Gene Transfer Techniques
/
Nanoparticles
Limits:
Humans
Language:
En
Year:
2011
Type:
Article