[Identification of medical Dendrobium herbs by ISSR marker].

Yang, Li-Chang; Deng, Hui; Yi, Yin; Zhang, Xi-Min; Wang, Ya-Zhu; Lin, Jun-Qing

Yang, Li-Chang; Deng, Hui; Yi, Yin; Zhang, Xi-Min; Wang, Ya-Zhu; Lin, Jun-Qing.

Affiliation

Yang LC; School of Sciences, Guizhou Normal University, Guiyang 550001, China.

Zhong Yao Cai ; 33(12): 1841-4, 2010 Dec.

Article in Zh | MEDLINE | ID: mdl-21548356

ABSTRACT

ABSTRACT

OBJECTIVE:

A molecular biology method was studied to identify medical Dendrobium and to provide a method for quality control of these plants.

METHOD:

ISSR primer was screened through ISSR-PCR reaction according to its gene resolving power, and digital barcodes were established for identification.

RESULTS:

Screening 2 primers which Rp value above 8, This primers can identify medical Dendrobium from 6 kinds 8 groups.

CONCLUSION:

ISSR molecular maker technology is useful for identifying species and habitats of medical Dendrobium plants.

Subject(s)

DNA, Plant/genetics; Dendrobium/classification; Dendrobium/genetics; Plants, Medicinal/genetics; Repetitive Sequences, Nucleic Acid; DNA Fingerprinting; DNA Primers; Genetic Markers; Genetics, Population; Phylogeny; Plant Leaves/genetics; Plants, Medicinal/classification; Polymerase Chain Reaction; Polymorphism, Genetic; Species Specificity

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Database: MEDLINE Main subject: Plants, Medicinal / Repetitive Sequences, Nucleic Acid / DNA, Plant / Dendrobium Type of study: Diagnostic_studies Language: Zh Year: 2010 Type: Article

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