Improved expression of secretory and trimeric proteins in mammalian cells via the introduction of a new trimer motif and a mutant of the tPA signal sequence.

Wang, Jia-Ye; Song, Wen-Ting; Li, Yan; Chen, Wen-Jiang; Yang, Dan; Zhong, Guo-Cai; Zhou, Hai-Zhou; Ren, Cai-Yun; Yu, Hao-Tong; Ling, Hong

Wang, Jia-Ye; Song, Wen-Ting; Li, Yan; Chen, Wen-Jiang; Yang, Dan; Zhong, Guo-Cai; Zhou, Hai-Zhou; Ren, Cai-Yun; Yu, Hao-Tong; Ling, Hong.

Affiliation

Wang JY; Department of Microbiology, Harbin Medical University, 194 Xuefu Road, Harbin, Heilongjiang 150081, China.

Appl Microbiol Biotechnol ; 91(3): 731-40, 2011 Aug.

Article in En | MEDLINE | ID: mdl-21556920

Subject(s)

Amino Acid Motifs/immunology; Multiprotein Complexes; Protein Sorting Signals/genetics; Tissue Plasminogen Activator/genetics; Amino Acid Sequence; Enzyme-Linked Immunosorbent Assay; HEK293 Cells; HIV/genetics; HIV/metabolism; Hemagglutinin Glycoproteins, Influenza Virus/genetics; Hemagglutinin Glycoproteins, Influenza Virus/immunology; Hemagglutinin Glycoproteins, Influenza Virus/metabolism; Humans; Influenza A Virus, H3N2 Subtype/genetics; Influenza A Virus, H3N2 Subtype/metabolism; Mutation; Protein Structure, Secondary; Recombinant Fusion Proteins/genetics; Recombinant Fusion Proteins/immunology; Tissue Plasminogen Activator/immunology; Vaccines, DNA/immunology; Vaccines, Subunit/immunology; gag Gene Products, Human Immunodeficiency Virus/genetics; gag Gene Products, Human Immunodeficiency Virus/immunology; gag Gene Products, Human Immunodeficiency Virus/metabolism

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Full text: 1 Database: MEDLINE Main subject: Protein Sorting Signals / Tissue Plasminogen Activator / Amino Acid Motifs / Multiprotein Complexes Limits: Humans Language: En Year: 2011 Type: Article

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