Coelomic fluid: a noninvasive source of DNA in earthworms.
Mol Ecol Resour
; 11(4): 645-9, 2011 Jul.
Article
in En
| MEDLINE
| ID: mdl-21676195
ABSTRACT
To investigate whether coelomic fluid secreted by earthworms can be a noninvasive source of DNA, we amplified and sequenced DNA extracted from the coelomic fluid and muscle tissue of eight worms. The sequences obtained using DNA extracted from both sources were identical. All cytochrome c oxidase subunit I (COI) mitochondrial DNA sequences, including those retrieved from GenBank, formed a monophyletic group of Metaphire sieboldi. The results indicate that we successfully extracted total DNA from coelomic fluid secreted by earthworm.
Full text:
1
Database:
MEDLINE
Main subject:
Oligochaeta
/
DNA, Mitochondrial
Limits:
Animals
Language:
En
Year:
2011
Type:
Article