MiR-140 is co-expressed with Wwp2-C transcript and activated by Sox9 to target Sp1 in maintaining the chondrocyte proliferation.
FEBS Lett
; 585(19): 2992-7, 2011 Oct 03.
Article
in En
| MEDLINE
| ID: mdl-21872590
ABSTRACT
MiR-140 is a microRNA specially involved in chondrogenesis and osteoarthritis pathogenesis. However, its transcriptional regulation and target genes in cartilage development are not fully understood. Here we detected that miR-140 was uniquely expressed in chondrocyte and suppressed by Wnt/ß-catenin signalling. The miR-140 primary transcript was an intron-retained RNA co-expressed with Wwp2-C isoform, which was directly induced by Sox9 through binding to the intron 10 of Wwp2 gene. Knockdown of miR-140 in limb bud micromass cultures resulted in arrest of chondrogenic proliferation. Sp1, the activator of the cell cycle regulator p15(INK4b), was identified as a target of miR-140 in maintaining the chondrocyte proliferation. Collectively, our findings expand our understanding of the transcriptional regulation and the chondrogenic role of miR-140 in chondrogenesis.
Full text:
1
Database:
MEDLINE
Main subject:
Sp1 Transcription Factor
/
Chondrocytes
/
MicroRNAs
/
Ubiquitin-Protein Ligases
/
Cell Proliferation
/
SOX9 Transcription Factor
Type of study:
Prognostic_studies
Limits:
Animals
/
Humans
Language:
En
Year:
2011
Type:
Article