How to confirm C.E.R.A. doping in athletes' blood?
Forensic Sci Int
; 213(1-3): 101-3, 2011 Dec 10.
Article
in En
| MEDLINE
| ID: mdl-21889277
ABSTRACT
C.E.R.A. (Continuous Erythropoietin Receptor Activator) is a new third-generation erythropoiesis-stimulating agent that has recently been linked with abuse in endurance sports. The anti-doping community rapidly reacted by releasing a high-throughput screening ELISA allowing the detection of C.E.R.A. doping in athletes' blood. In order to return adverse analytical findings, anti-doping laboratories, however, need, as far as possible, to confirm the presence of the drug in athletes' samples through orthogonal methods. This article focuses on the comparison of 2 proposed confirmation assays based on gel electrophoresis that were coupled with a new sample immunopurification method. IEF, the classical method used to target erythropoietin (EPO) and its recombinant analogues in athletes' samples, and SARKOSYL-PAGE were applied to the plasma samples of subjects having received a single injection of C.E.R.A. It was demonstrated that SARKOSYL-PAGE was at least 6 times more sensitive than IEF, with comparable specificity. A longer detection window coupled with easier interpretation criteria led us to recommend the use of SARKOSYL-PAGE to confirm C.E.R.A. presence in athletes' blood.
Full text:
1
Database:
MEDLINE
Main subject:
Substance Abuse Detection
/
Erythropoietin
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Doping in Sports
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Electrophoresis, Polyacrylamide Gel
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Hematinics
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Isoelectric Focusing
Limits:
Adult
/
Humans
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Male
Language:
En
Year:
2011
Type:
Article