Characterization of MspNI (G/GWCC) and MspNII (R/GATCY), novel thermostable Type II restriction endonucleases from Meiothermus sp., isoschizomers of AvaII and BstYI.

Gupta, Richa; Xu, Shuang-Yong; Sharma, Prince; Capalash, Neena

Gupta, Richa; Xu, Shuang-Yong; Sharma, Prince; Capalash, Neena.

Affiliation

Gupta R; Department of Biotechnology, Panjab University, Chandigarh, 160014, India.

Mol Biol Rep ; 39(5): 5607-14, 2012 May.

Article in En | MEDLINE | ID: mdl-22189541

Subject(s)

Bacterial Proteins/metabolism; Deoxyribonucleases, Type II Site-Specific/metabolism; Temperature; Thermus/enzymology; Base Sequence; Deoxyribonucleases, Type II Site-Specific/isolation & purification; Electrophoresis, Polyacrylamide Gel; Enzyme Stability; Methylation; Plasmids/metabolism; Restriction Mapping; Silver Staining

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Full text: 1 Database: MEDLINE Main subject: Temperature / Thermus / Bacterial Proteins / Deoxyribonucleases, Type II Site-Specific Language: En Year: 2012 Type: Article

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Full text: 1 Database: MEDLINE Main subject: Temperature / Thermus / Bacterial Proteins / Deoxyribonucleases, Type II Site-Specific Language: En Year: 2012 Type: Article