Development of BMP7-producing human cells, using a third generation lentiviral gene delivery system.

Chitty, David W; Tremblay, Roger G; Ribecco-Lutkiewicz, Maria; Haukenfrers, Julie; Zurakowski, Bogdan; Massie, Bernard; Sikorska, Marianna; Bani-Yaghoub, Mahmud

Chitty, David W; Tremblay, Roger G; Ribecco-Lutkiewicz, Maria; Haukenfrers, Julie; Zurakowski, Bogdan; Massie, Bernard; Sikorska, Marianna; Bani-Yaghoub, Mahmud.

Affiliation

Chitty DW; Neurogenesis and Brain Repair Group, Neurobiology Program, Institute for Biological Sciences, National Research Council Canada, Ottawa, ON K1A 0R6, Canada.

J Neurosci Methods ; 205(1): 17-27, 2012 Mar 30.

Article in En | MEDLINE | ID: mdl-22209770

Subject(s)

Bone Morphogenetic Protein 7/biosynthesis; Gene Transfer Techniques; Lentivirus/genetics; Transfection/methods; Amniotic Fluid/cytology; Animals; Blotting, Western; Cell Differentiation/genetics; Cell Differentiation/physiology; Cell Line; Cells, Cultured; DNA, Complementary/administration & dosage; DNA, Complementary/genetics; Enzyme-Linked Immunosorbent Assay; Female; Flow Cytometry; Green Fluorescent Proteins; Humans; Immunohistochemistry; Injections, Intraventricular; Mice; Neurons/metabolism; Neurons/physiology; Pregnancy; Smad Proteins/biosynthesis; Smad Proteins/genetics; Transduction, Genetic

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Full text: 1 Database: MEDLINE Main subject: Transfection / Gene Transfer Techniques / Lentivirus / Bone Morphogenetic Protein 7 Limits: Animals / Female / Humans / Pregnancy Language: En Year: 2012 Type: Article

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