A technique for the intra-gastric administration of live larvae of Anisakis simplex in mice.
Exp Parasitol
; 130(3): 285-7, 2012 Mar.
Article
in En
| MEDLINE
| ID: mdl-22269442
ABSTRACT
To understand the mechanisms of infection and to attempt to simulate human infection by the Anisakidae family, many in vivo experimental approaches have been developed. The aim was to develop and present a technique for the induction of an oral infection through the use of an intra-gastric gavage of live Anisakis simplex in mice. A commercial pediatric gastric tube (No. 4) was cut longitudinally to produce a 3-cm slit at the distal end where the larva was placed to then be administered to the stomach of the mouse. There were no abnormal clinical complications before, during or after the procedure. In conclusion oral infection through the direct delivery of larvae in the stomach is simple and effective.
Full text:
1
Database:
MEDLINE
Main subject:
Parasitology
/
Stomach
/
Anisakis
/
Anisakiasis
/
Disease Models, Animal
Type of study:
Observational_studies
Limits:
Animals
Language:
En
Year:
2012
Type:
Article