Delivery of Bordetella pertussis adenylate cyclase toxin to target cells via outer membrane vesicles.
FEBS Lett
; 586(4): 459-65, 2012 Feb 17.
Article
in En
| MEDLINE
| ID: mdl-22289177
ABSTRACT
Bordetella pertussis adenylate cyclase toxin (ACT) intoxicates cells by producing intracellular cAMP. B. pertussis outer membrane vesicles (OMV) contain ACT on their surface (OMV-ACT), but the properties of OMV-ACT were previously unknown. We found that B. pertussis in the lung from a fatal pertussis case contains OMV, suggesting an involvement in pathogenesis. OMV-ACT and ACT intoxicate cells with and without the toxin's receptor CD11b/CD18. Intoxication by ACT is blocked by antitoxin and anti-CD11b antibodies, but not by cytochalasin-D; in contrast, OMV-ACT is unaffected by either antibody and blocked by cytochalasin-D. Thus OMV-ACT can deliver ACT by processes distinct from those of ACT alone.
Full text:
1
Database:
MEDLINE
Main subject:
Bordetella pertussis
/
Adenylate Cyclase Toxin
Type of study:
Etiology_studies
Limits:
Animals
/
Humans
Language:
En
Year:
2012
Type:
Article