Uromodulin and α(1)-antitrypsin urinary peptide analysis to differentiate glomerular kidney diseases.
Kidney Blood Press Res
; 35(5): 314-25, 2012.
Article
in En
| MEDLINE
| ID: mdl-22399069
ABSTRACT
BACKGROUND/AIMS:
Glomerular kidney disease (GKD) is suspected in patients based on proteinuria, but its diagnosis relies primarily on renal biopsy. We used urine peptide profiling as a noninvasive means to link GKD-associated changes to each glomerular entity.METHODS:
Urinary peptide profiles of 60 biopsy-proven glomerular patients and 14 controls were analyzed by combining magnetic bead peptide enrichment, MALDI-TOF MS analysis, and ClinProTools v2.0 to select differential peptides. Tentative identification of the differential peptides was carried out by HPLC-MS/MS.RESULTS:
The HPLC-MS/MS results suggest that uromodulin (UMOD; m/z 1682, 1898 and 1913) and α(1)-antitrypsin (A1AT; m/z 1945, 2392 and 2505) are differentially expressed urinary peptides that distinguish between GKD patients and healthy subjects. Low UMOD and high A1AT peptide abundance was observed in 80-92% of patients with GKD. Proliferative forms of GKD were distinguished from nonproliferative forms, based on a combination of UMOD and A1AT peptides. Nonproliferative forms correlated with higher A1AT peptide levels - focal segmental glomerulosclerosis was linked more closely to high levels of the m/z 1945 peptide than minimal change disease.CONCLUSION:
We describe a workflow - urinary peptide profiling coupled with histological findings - that can be used to distinguish GKD accurately and noninvasively, particularly its nonproliferative forms.
Full text:
1
Database:
MEDLINE
Main subject:
Alpha 1-Antitrypsin
/
Protein Array Analysis
/
Uromodulin
/
Glomerulonephritis
Type of study:
Diagnostic_studies
/
Prognostic_studies
Limits:
Adult
/
Female
/
Humans
/
Male
/
Middle aged
Language:
En
Year:
2012
Type:
Article