Localization of residues in a novel DNA-binding domain of DmSNAP43 required for DmSNAPc DNA-binding activity.
FEBS Lett
; 586(6): 841-6, 2012 Mar 23.
Article
in En
| MEDLINE
| ID: mdl-22449969
ABSTRACT
Transcription of snRNA genes depends upon the recognition of the proximal sequence element (PSE) by the snRNA activating protein complex SNAPc. In Drosophila melanogaster, all subunits of DmSNAPc (DmSNAP43, DmSNAP50, and DmSNAP190) are required for PSE-binding activity. Previous work demonstrated that a non-canonical DmSNAP43 domain bounded by residues 193-272 was essential for DmSNAPc to bind to the PSE. In this study, the contribution of amino acid residues within this domain to DNA binding by DmSNAPc was investigated by alanine-scanning mutagenesis. The results have identified two clusters of residues within this domain required for the sequence-specific DNA-binding activity of DmSNAPc.
Full text:
1
Database:
MEDLINE
Main subject:
DNA
/
Protein Subunits
/
Drosophila Proteins
/
DNA-Binding Proteins
Limits:
Animals
/
Humans
Language:
En
Year:
2012
Type:
Article