Comparison of two eukaryotic systems for the expression of VP6 protein of rotavirus specie A: transient gene expression in HEK293-T cells and insect cell-baculovirus system.

da Silva Junior, Haroldo Cid; da Silva E Mouta Junior, Sérgio; de Mendonça, Marcos César Lima; de Souza Pereira, Mirian Claudia; da Rocha Nogueira, Alanderson; de Azevedo, Maria Luiza Borges; Leite, José Paulo Gagliardi; de Moraes, Márcia Terezinha Baroni

da Silva Junior, Haroldo Cid; da Silva E Mouta Junior, Sérgio; de Mendonça, Marcos César Lima; de Souza Pereira, Mirian Claudia; da Rocha Nogueira, Alanderson; de Azevedo, Maria Luiza Borges; Leite, José Paulo Gagliardi; de Moraes, Márcia Terezinha Baroni.

Affiliation

da Silva Junior HC; Laboratório de Virologia Comparada e Ambiental, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz. Av. Brasil, 4365, Pavilhão Hélio & Peggy Pereira, Rio de Janeiro, RJ 21040-360, Brazil. haroldo.cid@bio.fiocruz.br

Biotechnol Lett ; 34(9): 1623-7, 2012 Sep.

Article in En | MEDLINE | ID: mdl-22576283

Subject(s)

Antigens, Viral/biosynthesis; Antigens, Viral/isolation & purification; Biotechnology/methods; Capsid Proteins/biosynthesis; Capsid Proteins/isolation & purification; Gene Expression; Animals; Antigens, Viral/genetics; Baculoviridae/genetics; Capsid Proteins/genetics; Cell Line; Genetic Vectors; HEK293 Cells; Humans; Insecta; Proteolysis; Recombinant Proteins/biosynthesis; Recombinant Proteins/genetics; Recombinant Proteins/isolation & purification; Rotavirus/genetics

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Full text: 1 Database: MEDLINE Main subject: Biotechnology / Gene Expression / Capsid Proteins / Antigens, Viral Limits: Animals / Humans Language: En Year: 2012 Type: Article

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