A GFP-based bacterial biosensor with chromosomally integrated sensing cassette for quantitative detection of Hg(II) in environment.
J Environ Sci (China)
; 24(5): 963-8, 2012.
Article
in En
| MEDLINE
| ID: mdl-22893977
ABSTRACT
A mercury biosensor was constructed by integrating biosensor genetic elements into E. coli JM109 chromosome in a single copy number, using the attP/attB recombination mechanism of lambda phage. The genetic elements used include a regulatory protein gene (merR) along with operator/promoter (O/P) derived from the mercury resistance operon from pDU1358 plasmid of Serratia marcescens. The expression of reporter gene gfp is also controlled by merR/O/P. Integration of the construct into the chromosome was done to increase the stability and precision of the biosensor. This biosensor could detect Hg(II) ions in the concentration range of 100-1700 nmol/L, and manifest the result as the expression of GFP. The GFP expression was significantly different (P < or = 0.05) for each concentration of inducing Hg(II) ions in the detection range, which reduces the chances of misinterpretation of results. A model using regression method was also derived for the quantification of the concentration of Hg(II) in water samples.
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Database:
MEDLINE
Main subject:
Biosensing Techniques
/
Mutagenesis, Insertional
/
Chromosomes, Bacterial
/
Green Fluorescent Proteins
/
Environmental Microbiology
/
Escherichia coli
/
Mercury
Type of study:
Diagnostic_studies
/
Prognostic_studies
Language:
En
Year:
2012
Type:
Article