Incorporation of 4'-C-aminomethyl-2'-O-methylthymidine into DNA by thermophilic DNA polymerases.
Chem Commun (Camb)
; 48(77): 9619-21, 2012 Oct 07.
Article
in En
| MEDLINE
| ID: mdl-22908130
ABSTRACT
The dual modified nucleotide 4'-C-aminomethyl-2'-O-methylthymidine 5'-triphosphate was synthesized and enzymatically incorporated into DNA by the thermophilic DNA polymerases Pfu and Therminator III. The dual ribose modification imparted increased exonuclease resistance to DNA compared to the well-known 2'-O-methyl modification.
Full text:
1
Database:
MEDLINE
Main subject:
Temperature
/
Thymidine
/
DNA
/
DNA-Directed DNA Polymerase
Language:
En
Year:
2012
Type:
Article