Oleic acid mitigates TNF-α-induced oxidative stress in rat cardiomyocytes.
Mol Cell Biochem
; 372(1-2): 75-82, 2013 Jan.
Article
in En
| MEDLINE
| ID: mdl-22961439
ABSTRACT
Some of the effects of tumor necrosis factor alpha (TNF-α) are suggested to be mediated by oxidative stress. It has also been reported that dietary supplements of olive oil result in a reduction in LDL, oxidative stress, and blood pressure and these effects are attributed to oleic acid (OA)-a major component of olive oil. The objective of this study was to examine the beneficial effects of OA against TNF-α-induced oxidative stress and cardiomyocytes injury. Isolated cardiomyocytes from adult rat hearts were treated as follows (A) control; (B) OA (50 µM); (C) TNF-α (10 ng/ml); and (D) TNF-α + OA. After 4 h of the treatment, cells were assessed for oxidative stress, cellular damage, viability, and apoptosis. Cardiomyocytes treated with TNF-α showed a significant increase (P < 0.05) in reactive oxygen species, decrease in the viability of cells, and increase in creatine kinase release. All these TNF-α-induced changes were prevented by OA. TNF-α also caused a significant increase in the expression of apoptotic proteins Bax, Caspase 3 and PARP cleavage, Bnip3, and TGF-ß , whereas OA modulated these changes. It is suggested that TNF-α induced oxidative stress mediates cardiomyocyte cell damage which is prevented by OA.
Full text:
1
Database:
MEDLINE
Main subject:
Tumor Necrosis Factor-alpha
/
Oxidative Stress
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Oleic Acid
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Myocytes, Cardiac
/
Antioxidants
Limits:
Animals
Language:
En
Year:
2013
Type:
Article