Optimization of a GCaMP calcium indicator for neural activity imaging.

Akerboom, Jasper; Chen, Tsai-Wen; Wardill, Trevor J; Tian, Lin; Marvin, Jonathan S; Mutlu, Sevinç; Calderón, Nicole Carreras; Esposti, Federico; Borghuis, Bart G; Sun, Xiaonan Richard; Gordus, Andrew; Orger, Michael B; Portugues, Ruben; Engert, Florian; Macklin, John J; Filosa, Alessandro; Aggarwal, Aman; Kerr, Rex A; Takagi, Ryousuke; Kracun, Sebastian; Shigetomi, Eiji; Khakh, Baljit S; Baier, Herwig; Lagnado, Leon; Wang, Samuel S-H; Bargmann, Cornelia I; Kimmel, Bruce E; Jayaraman, Vivek; Svoboda, Karel; Kim, Douglas S; Schreiter, Eric R; Looger, Loren L

Akerboom, Jasper; Chen, Tsai-Wen; Wardill, Trevor J; Tian, Lin; Marvin, Jonathan S; Mutlu, Sevinç; Calderón, Nicole Carreras; Esposti, Federico; Borghuis, Bart G; Sun, Xiaonan Richard; Gordus, Andrew; Orger, Michael B; Portugues, Ruben; Engert, Florian; Macklin, John J; Filosa, Alessandro; Aggarwal, Aman; Kerr, Rex A; Takagi, Ryousuke; Kracun, Sebastian; Shigetomi, Eiji; Khakh, Baljit S; Baier, Herwig; Lagnado, Leon; Wang, Samuel S-H; Bargmann, Cornelia I; Kimmel, Bruce E; Jayaraman, Vivek; Svoboda, Karel; Kim, Douglas S; Schreiter, Eric R; Looger, Loren L.

Affiliation

Akerboom J; Howard Hughes Medical Institute, Janelia Farm Research Campus, Ashburn, Virginia 20147, USA.

J Neurosci ; 32(40): 13819-40, 2012 Oct 03.

Article in En | MEDLINE | ID: mdl-23035093

Subject(s)

Calcium Signaling; Fluorescent Dyes/chemistry; Fluorometry/methods; Green Fluorescent Proteins/chemistry; Neuroimaging/methods; Neurons/chemistry; Peptides/chemistry; Synaptic Transmission; Animals; Astrocytes/chemistry; Astrocytes/ultrastructure; Caenorhabditis elegans; Crystallography, X-Ray; Drosophila melanogaster/growth & development; Female; Fluorescent Dyes/analysis; Genes, Synthetic; Genetic Vectors; Green Fluorescent Proteins/analysis; Green Fluorescent Proteins/genetics; Green Fluorescent Proteins/isolation & purification; HEK293 Cells/chemistry; HEK293 Cells/ultrastructure; Hippocampus/chemistry; Hippocampus/cytology; Humans; Larva; Lasers; Mice; Models, Molecular; Mutagenesis, Site-Directed; Neuromuscular Junction/chemistry; Neuromuscular Junction/ultrastructure; Neurons/physiology; Neurons/ultrastructure; Neuropil/chemistry; Neuropil/physiology; Neuropil/ultrastructure; Olfactory Receptor Neurons/chemistry; Olfactory Receptor Neurons/physiology; Olfactory Receptor Neurons/ultrastructure; Peptides/analysis; Peptides/genetics; Photic Stimulation; Protein Conformation; Rats; Recombinant Fusion Proteins/analysis; Recombinant Fusion Proteins/chemistry; Recombinant Fusion Proteins/genetics; Retinal Bipolar Cells/chemistry; Retinal Bipolar Cells/physiology; Retinal Bipolar Cells/ultrastructure; Zebrafish/growth & development

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Full text: 1 Database: MEDLINE Main subject: Peptides / Synaptic Transmission / Calcium Signaling / Green Fluorescent Proteins / Neuroimaging / Fluorescent Dyes / Fluorometry / Neurons Language: En Year: 2012 Type: Article

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