Development of a peptide nucleic acid probe to Trichosporon species and identification of trichosporonosis by use of in situ hybridization in formalin-fixed and paraffin-embedded (FFPE) sections.
J Clin Microbiol
; 51(1): 295-8, 2013 Jan.
Article
in En
| MEDLINE
| ID: mdl-23100341
ABSTRACT
In order to identify Trichosporon species in formalin-fixed and paraffin-embedded sections from which visual discrimination of non-glabrata Candida species is mostly ineffective but critical for the choice of antifungals, we tested the usefulness of a newly designed peptide nucleic acid probe (PNA) for in situ hybridization (ISH). Results confirmed the usefulness of ISH with our PNA probe in identifying Trichosporon species from Candida albicans.
Full text:
1
Database:
MEDLINE
Main subject:
Trichosporon
/
Oligonucleotide Probes
/
In Situ Hybridization
/
Peptide Nucleic Acids
/
Pathology, Molecular
/
Trichosporonosis
Type of study:
Diagnostic_studies
/
Evaluation_studies
/
Prognostic_studies
Limits:
Humans
Language:
En
Year:
2013
Type:
Article