Engineering of substrate specificity of D-amino acid oxidase from the yeast Trigonopsis variabilis: directed mutagenesis of Phe258 residue.

Komarova, N V; Golubev, I V; Khoronenkova, S V; Chubar', T A; Tishkov, V I

Komarova, N V; Golubev, I V; Khoronenkova, S V; Chubar', T A; Tishkov, V I.

Affiliation

Komarova NV; Innovations and High Technologies MSU Ltd., 109559 Moscow, Russia.

Biochemistry (Mosc) ; 77(10): 1181-9, 2012 Oct.

Article in En | MEDLINE | ID: mdl-23157298

Subject(s)

D-Amino-Acid Oxidase/genetics; D-Amino-Acid Oxidase/metabolism; Yeasts/enzymology; Amino Acid Substitution; D-Amino-Acid Oxidase/chemistry; Drug Design; Drug Stability; Electrophoresis, Polyacrylamide Gel; Models, Molecular; Mutagenesis, Site-Directed; Mutation; Substrate Specificity; Temperature

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