High throughput genomic screen identifies multiple factors that promote cooperative Wnt signaling.
PLoS One
; 8(1): e55782, 2013.
Article
in En
| MEDLINE
| ID: mdl-23383281
ABSTRACT
Previous studies have demonstrated that certain Wnt ligands can promote high levels of cooperative signaling in a cell type specific manner. To explore the underlying mechanism of this cooperative Wnt signaling, we performed a high-throughput screen of more than 14,000 cDNAs to identify genes that promote cooperative Wnt signaling in the context of a single Wnt ligand, Wnt2. This screen identified several homeobox factors including Msx2, Nkx5.2, and Esx1, in addition to other factors known to promote Wnt signaling including Pias4. Generation of dominant-active or dominant-negative forms of Msx2 indicate that the mechanism by which homeobox factors cooperatively promote Wnt signaling is through their ability to repress gene transcription. These data identify a broad homeobox code, which acts to increase Wnt signaling through transcriptional repression.
Full text:
1
Database:
MEDLINE
Main subject:
Genomics
/
Wnt Proteins
/
Wnt Signaling Pathway
Type of study:
Prognostic_studies
Limits:
Humans
Language:
En
Year:
2013
Type:
Article