Development of a ligase detection reaction/CGE method using a LIF dual-channel detection system for direct identification of allelic composition of mutated DNA in a mixed population of excess wild-type DNA.

Hamada, Mariko; Shimase, Koji; Noda, Keiichi; Tsukagoshi, Kazuhiko; Hashimoto, Masahiko

Hamada, Mariko; Shimase, Koji; Noda, Keiichi; Tsukagoshi, Kazuhiko; Hashimoto, Masahiko.

Affiliation

Hamada M; Department of Chemical Engineering and Materials Science, Faculty of Science and Engineering, Doshisha University, Kyotanabe, Kyoto, Japan.

Electrophoresis ; 34(9-10): 1415-22, 2013 May.

Article in En | MEDLINE | ID: mdl-23463388

Subject(s)

DNA/genetics; Electrophoresis, Capillary/instrumentation; Genes, ras; Point Mutation; Alleles; DNA/metabolism; DNA Ligases/metabolism; Electrophoresis, Capillary/methods; Equipment Design; Fluorescence; Fluorescent Dyes/analysis; Lasers

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Full text: 1 Database: MEDLINE Main subject: DNA / Genes, ras / Point Mutation / Electrophoresis, Capillary Type of study: Diagnostic_studies Language: En Year: 2013 Type: Article

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