Genetic variability of Echinococcus granulosus from the Tibetan plateau inferred by mitochondrial DNA sequences.
Vet Parasitol
; 196(1-2): 179-83, 2013 Sep 01.
Article
in En
| MEDLINE
| ID: mdl-23523013
ABSTRACT
To analyse genetic variability and population structure, 84 isolates of Echinococcus granulosus (Cestoda Taeniidae) collected from various host species at different sites of the Tibetan plateau in China were sequenced for the whole mitochondrial nad1 (894 bp) and atp6 (513 bp) genes. The vast majority were classified as G1 genotype (n=82), and two samples from human patients in Sichuan province were identified as G3 genotype. Based on the concatenated sequences of nad1+atp6, 28 different haplotypes (NA1-NA28) were identified. A parsimonious network of the concatenated sequence haplotypes showed star-like features in the overall population, with NA1 as the major haplotype in the population networks. By AMOVA it was shown that variation of E. granulosus within the overall population was the main pattern of the total genetic variability. Neutrality indexes of the concatenated sequence (nad1+atp6) were computed by Tajima's D and Fu's Fs tests and showed high negative values for E. granulosus, indicating significant deviations from neutrality. FST and Nm values suggested that the populations were not genetically differentiated.
Key words
Full text:
1
Database:
MEDLINE
Main subject:
Genetic Variation
/
DNA, Mitochondrial
/
Echinococcus granulosus
/
Echinococcosis
Type of study:
Prognostic_studies
/
Risk_factors_studies
Limits:
Animals
/
Humans
Country/Region as subject:
Asia
Language:
En
Year:
2013
Type:
Article