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The PI3K/Akt pathway mediates the protection of SO(2) preconditioning against myocardial ischemia/reperfusion injury in rats.
Zhao, Man-man; Yang, Jin-yan; Wang, Xin-bao; Tang, Chao-shu; Du, Jun-bao; Jin, Hong-fang.
Affiliation
  • Zhao MM; Department of Pediatrics, Peking University First Hospital, Beijing 100034, China.
Acta Pharmacol Sin ; 34(4): 501-6, 2013 Apr.
Article in En | MEDLINE | ID: mdl-23524571
ABSTRACT

AIM:

To explore the mechanisms underlying the protection by SO2 preconditioning against rat myocardial ischemia/reperfusion (I/R) injury.

METHODS:

Male Wistar rats underwent 30-min left coronary artery ligation followed by 120-min reperfusion. An SO2 donor (1 µmol/kg) was intravenously injected 10 min before the ischemia, while LY294002 (0.3 mg/kg) was intravenously injected 30 min before the ischemia. Plasma activities of LDH and CK were measured with an automatic enzyme analyzer. Myocardial infarct size was detected using Evans-TTC method. The activities of caspase-3 and -9 in myocardium were assayed using a commercial kit, and the levels of p-Akt, Akt, PI3K and p-PI3K were examined with Western blotting.

RESULTS:

Pretreatment with SO2 significantly reduced the myocardial infarct size and plasma LDH and CK activities, as well as myocardial caspase-3 and -9 activities in the rats. Furthermore, the pretreatment significantly increased the expression levels of myocardial p-Akt and p-PI3K p85. Administration of the PI3K inhibitor LY294002 blocked all the effects induced by SO2 pretreatment.

CONCLUSION:

The results suggest that the PI3K/Akt pathway mediates the protective effects of SO2 preconditioning against myocardial I/R injury in rats.
Subject(s)

Full text: 1 Database: MEDLINE Main subject: Sulfur Dioxide / Myocardial Reperfusion Injury / Ischemic Preconditioning, Myocardial / Phosphatidylinositol 3-Kinases / Proto-Oncogene Proteins c-akt / Myocardium Limits: Animals Language: En Year: 2013 Type: Article

Full text: 1 Database: MEDLINE Main subject: Sulfur Dioxide / Myocardial Reperfusion Injury / Ischemic Preconditioning, Myocardial / Phosphatidylinositol 3-Kinases / Proto-Oncogene Proteins c-akt / Myocardium Limits: Animals Language: En Year: 2013 Type: Article