Effects of maternal nutrient restriction, intrauterine growth restriction, and glucocorticoid exposure on phosphoenolpyruvate carboxykinase-1 expression in fetal baboon hepatocytes in vitro.
J Med Primatol
; 42(4): 211-9, 2013 Aug.
Article
in En
| MEDLINE
| ID: mdl-23600855
ABSTRACT
BACKGROUND:
The objective of this study was to develop a cell culture system for fetal baboon hepatocytes and to test the hypotheses that (i) expression of the gluconeogenic enzyme phosphoenolpyruvate carboxykinase-1 (PEPCK-1) is upregulated in hepatocytes isolated from fetuses of nutrient-restricted mothers (MNR) compared with ad libitum-fed controls (CTR), and (ii) glucocorticoids stimulate PEPCK-1 expression.METHODS:
Hepatocytes from 0.9G CTR and MNR fetuses were isolated and cultured. PEPCK-1 protein and mRNA levels in hepatocytes were determined by Western blot and quantitative PCR, respectively.RESULTS:
Fetuses of MNR mothers were intrauterine growth restricted (IUGR). Feasibility of culturing 0.9G fetal baboon hepatocytes was demonstrated. PEPCK-1 protein levels were increased in hepatocytes isolated from IUGR fetuses, and PEPCK-1 mRNA expression was stimulated by glucocorticoids in fetal hepatocytes.CONCLUSIONS:
Cultured fetal baboon hepatocytes that retain their in vivo phenotype provide powerful in vitro tools to investigate mechanisms that regulate normal and programmed hepatic function.Key words
Full text:
1
Database:
MEDLINE
Main subject:
Papio
/
Phosphoenolpyruvate Carboxykinase (GTP)
/
Hepatocytes
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Fetal Growth Retardation
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Food Deprivation
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Glucocorticoids
Limits:
Animals
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Pregnancy
Language:
En
Year:
2013
Type:
Article