Mapping the conformational space accessible to BACE2 using surface mutants and cocrystals with Fab fragments, Fynomers and Xaperones.

Banner, David W; Gsell, Bernard; Benz, Jörg; Bertschinger, Julian; Burger, Dominique; Brack, Simon; Cuppuleri, Simon; Debulpaep, Maja; Gast, Alain; Grabulovski, Dragan; Hennig, Michael; Hilpert, Hans; Huber, Walter; Kuglstatter, Andreas; Kusznir, Eric; Laeremans, Toon; Matile, Hugues; Miscenic, Christian; Rufer, Arne C; Schlatter, Daniel; Steyaert, Jan; Stihle, Martine; Thoma, Ralf; Weber, Martin; Ruf, Armin

Banner, David W; Gsell, Bernard; Benz, Jörg; Bertschinger, Julian; Burger, Dominique; Brack, Simon; Cuppuleri, Simon; Debulpaep, Maja; Gast, Alain; Grabulovski, Dragan; Hennig, Michael; Hilpert, Hans; Huber, Walter; Kuglstatter, Andreas; Kusznir, Eric; Laeremans, Toon; Matile, Hugues; Miscenic, Christian; Rufer, Arne C; Schlatter, Daniel; Steyaert, Jan; Stihle, Martine; Thoma, Ralf; Weber, Martin; Ruf, Armin.

Affiliation

Banner DW; pRED Pharma Research and Early Development, Small Molecule Research, Discovery Technologies, F. Hoffmann-La Roche Ltd, 4070 Basel, Switzerland.

Acta Crystallogr D Biol Crystallogr ; 69(Pt 6): 1124-37, 2013 Jun.

Article in En | MEDLINE | ID: mdl-23695257

Subject(s)

Amyloid Precursor Protein Secretases/chemistry; Aspartic Acid Endopeptidases/chemistry; Immunoglobulin Fab Fragments/chemistry; Insulin-Secreting Cells/enzymology; Amyloid Precursor Protein Secretases/genetics; Amyloid Precursor Protein Secretases/metabolism; Animals; Area Under Curve; Aspartic Acid Endopeptidases/genetics; Aspartic Acid Endopeptidases/metabolism; Catalytic Domain; Crystallization; Humans; Immunoglobulin Fab Fragments/metabolism; Insulin-Secreting Cells/metabolism; Mice; Models, Molecular; Mutagenesis; Protein Conformation; Surface Plasmon Resonance; X-Ray Diffraction

Key words

antibodies; drug discovery and design; protein crystallization

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Full text: 1 Database: MEDLINE Main subject: Immunoglobulin Fab Fragments / Aspartic Acid Endopeptidases / Insulin-Secreting Cells / Amyloid Precursor Protein Secretases Type of study: Prognostic_studies Limits: Animals / Humans Language: En Year: 2013 Type: Article

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